Description

Part I: Introduction to biology (5h50 CM)

- Basis of Biology, Immunology and Microbiology

Part II: Bioanalytical chemistry (21h: 17h30 CM, 3h30 TD)

- Purification of biomolecules (liquid chromatography, gel electrophoresis)
- Characterization of proteins using MS-based approaches (bottom-up, top-down, data-dependent acquisition, data-independent acquisition)
- Development of proteomic strategies for global and targeted analyses, including protein quantification
- Structural proteomics (native MS, HDX, crosslinking)
- Case studies: analysis of publications

Part III: Contribution of miniaturization for biomolecules (11h40: 10h30 CM, 1h10 TD)

- Introduction. Interests and specificities of nanometric scale for separation methods. Transport mode, separation modes and dispersion effects.
- Last insights in nanometric scale for chromatography, from micro to nano flow rate. Evolution of new stationary phases, i.e. monoliths.
- Introduction to electrophoretic separation. From 2D Gel to capillary electrophoresis. Overview of the different detection modes (UV, Fluorescent, MS)
- Lab on chip, the future of miniaturization for separation methods.
- Example of miniaturization for health application.
 

MCC

Les épreuves indiquées respectent et appliquent le règlement de votre formation, disponible dans l'onglet Documents de la description de la formation.

Régime d'évaluation

CT (Contrôle terminal, mêlé de contrôle continu)

Coefficient

6.0

Évaluation initiale / Session principale - Épreuves

Seconde chance / Session de rattrapage - Épreuves