Description

• Purification of biomolecules using liquid chromatography (SEC, IEC, HIC, RP, HILIC, IMAC, affinity). Preparative and miniaturized scales.
• Purification of biomolecules using electrophoresis in denaturating or native conditions. (1D, 2D, off gel).
• Contribution of mass spectrometry to the characterization of biomolecules: ionization sources and analyzers for biomolecules, interpretation of mass spectra MS and MS/MS of biomolecules, identification of proteins from mass spectrometry data.
• Shotgun and targeted proteomic strategies.
• Strategies of relative and absolute quantification of proteins in complex mixtures.
• Supramolecular mass spectrometry.

Compétences visées

• Understanding of the different approaches for the characterization and the quantification of biomolecules.
• Design of a convenient proteomic strategy.

Contacts

Responsable(s) de l'enseignement

• Laurence Sabatier

MCC

Les épreuves indiquées respectent et appliquent le règlement de votre formation, disponible dans l'onglet Documents de la description de la formation.

Régime d'évaluation

CT (Contrôle terminal, mêlé de contrôle continu)

Coefficient

3.0